2OL0
High Resolution Crystal Structures of Vaccinia Virus dUTPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-06-20 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 65 |
| Unit cell lengths | 119.660, 119.660, 50.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.580 - 2.100 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2okd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.188 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0011) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.940 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.078 | 0.347 |
| Number of reflections | 24014 | |
| <I/σ(I)> | 10.2 | 3.4 |
| Completeness [%] | 99.5 | 99 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | 20% PEG2000 MME, 0.1M Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7.00 |
