2OJU

X-ray structure of complex of human cyclophilin J with cyclosporin A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	293
Detector technology	CCD
Collection date	2005-02-26
Spacegroup name	P 31 2 1
Unit cell lengths	64.387, 64.387, 200.943
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.193
Rwork	0.193
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ok3
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.076	0.406
Number of reflections	19272
Completeness [%]	97.6	83.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.4		0.1MOL/L TRIS-HCL(PH=7.4), 6%(V/V) DMSO, 18%(W/V) PEG8000, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293.0K