2OJH
The structure of putative TolB from Agrobacterium tumefaciens
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-03 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97918, 0.97935 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.714, 56.159, 48.332 |
| Unit cell angles | 90.00, 107.95, 90.00 |
Refinement procedure
| Resolution | 35.500 - 1.850 |
| R-factor | 0.17224 |
| Rwork | 0.170 |
| R-free | 0.21594 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.462 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.500 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 29685 | |
| <I/σ(I)> | 9.7 | 3.4 |
| Completeness [%] | 93.8 | 70.1 |
| Redundancy | 6.5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Sodium cacodylate pH 6.5, 0.2M Magnesium acetate, 20% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
