2OIU
L1 Ribozyme Ligase circular adduct
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9840 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.290, 100.018, 71.930 |
| Unit cell angles | 90.00, 104.42, 90.00 |
Refinement procedure
| Resolution | 31.340 - 2.600 |
| R-factor | 0.2065 |
| Rwork | 0.203 |
| R-free | 0.23776 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.976 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0012) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.044 | 0.467 |
| Number of reflections | 19071 | |
| <I/σ(I)> | 20.2 | 2.5 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 1.8M lithium sulfate, 10mM magnesium sulfate, 50mM sodium cacodylate (pH 6.0), 100mM sodium chloride, 60mM magnesium chloride, 30mM tris (pH 7.6), VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | lithium sulfate | ||
| 10 | 1 | 2 | sodium chloride | ||
| 11 | 1 | 2 | magnesium chloride | ||
| 12 | 1 | 2 | tris | ||
| 2 | 1 | 1 | magnesium sulfate | ||
| 3 | 1 | 1 | sodium cacodylate | ||
| 4 | 1 | 1 | sodium chloride | ||
| 5 | 1 | 1 | magnesium chloride | ||
| 6 | 1 | 1 | tris | ||
| 7 | 1 | 2 | lithium sulfate | ||
| 8 | 1 | 2 | magnesium sulfate | ||
| 9 | 1 | 2 | sodium cacodylate |
