2OIB
Crystal structure of IRAK4 kinase domain apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2005-04-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.066, 138.750, 87.942 |
| Unit cell angles | 90.00, 123.95, 90.00 |
Refinement procedure
| Resolution | 48.560 - 2.000 |
| R-factor | 0.22351 |
| Rwork | 0.221 |
| R-free | 0.26981 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uwh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.073 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 87970 | |
| <I/σ(I)> | 9.1 | 2.2 |
| Completeness [%] | 92.6 | 62.3 |
| Redundancy | 3.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.3M sodium malonate, 0.1M sodium acetate, 0.01M DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
