2OHH
Crystal Structure of coenzyme F420H2 oxidase (FprA), a diiron flavoprotein, active oxidized state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.740, 120.860, 92.690 |
| Unit cell angles | 90.00, 110.40, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.700 |
| R-factor | 0.18595 |
| Rwork | 0.184 |
| R-free | 0.21843 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Rugredoxin:NO/NO2 oxidoreductase from Moorella thermoacetica |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.871 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.770 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 156544 | |
| <I/σ(I)> | 9.9 | 2.3 |
| Completeness [%] | 99.2 | 98.9 |
| Redundancy | 4.6 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 283 | 0.001M DDT, 0.2M ammonium sulfate, 0.1M MES/KOH, 30% PEG MME 5000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
