2OGM
The crystal structure of the large ribosomal subunit from Deinococcus radiodurans complexed with the pleuromutilin derivative SB-571519
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Detector technology | CCD |
Collection date | 2006-03-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.033201 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 170.396, 405.835, 703.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.930 - 3.500 |
Rwork | 0.275 |
R-free | 0.33400 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.620 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.184 | 0.797 |
Number of reflections | 284085 | |
<I/σ(I)> | 6.3 | 1.5 |
Completeness [%] | 92.7 | 84.9 |
Redundancy | 4.9 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 291 | Ethanol, Dimethylhexanediol, MgCl2, HEPES, NH4Cl, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Ethanol | ||
10 | 1 | 2 | HEPES | ||
11 | 1 | 2 | NH4Cl | ||
2 | 1 | 1 | Dimethylhexanediol | ||
3 | 1 | 1 | MgCl2 | ||
4 | 1 | 1 | HEPES | ||
5 | 1 | 1 | NH4Cl | ||
6 | 1 | 1 | H2O | ||
7 | 1 | 2 | Ethanol | ||
8 | 1 | 2 | Dimethylhexanediol | ||
9 | 1 | 2 | MgCl2 |