2OG9
Crystal Structure of mandelate racemase/muconate lactonizing enzyme from Polaromonas sp. JS666
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97900 |
| Spacegroup name | I 4 |
| Unit cell lengths | 133.416, 133.416, 87.434 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.840 - 1.900 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.23000 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.380 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 60304 | |
| <I/σ(I)> | 18.3 | 3.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.1 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | VAPOR DIFFUSION, SITTING DROP |
