2OFR
1.00 A Crystal Structure Of V36A/D129A/L130A Mutant of Nitrophorin 4 From Rhodnius Prolixus Complexed With Nitric Oxide at pH 5.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.191, 42.637, 52.975 |
| Unit cell angles | 90.00, 94.12, 90.00 |
Refinement procedure
| Resolution | 9.470 - 1.000 |
| R-factor | 0.127 |
| Rwork | 0.126 |
| R-free | 0.14795 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1sxx |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.983 |
| Data scaling software | CrystalClear |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.850 | 1.040 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.100 | 0.390 |
| Number of reflections | 79668 | |
| <I/σ(I)> | 37 | 5.33 |
| Completeness [%] | 94.1 | 90.1 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 300 | ammonium phosphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
