2OF7
Structural Genomics, the crystal structure of a tetR-family transcriptional regulator from Streptomyces coelicolor A3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97902 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 75.101, 73.240, 48.082 |
| Unit cell angles | 90.00, 107.27, 90.00 |
Refinement procedure
| Resolution | 36.610 - 2.300 |
| R-factor | 0.23389 |
| Rwork | 0.231 |
| R-free | 0.29270 |
| Structure solution method | SAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.616 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.610 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 | 0.282 |
| Number of reflections | 10963 | |
| <I/σ(I)> | 28.5 | 3.3 |
| Completeness [%] | 98.8 | 91.6 |
| Redundancy | 4.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2M NH4(OAC), 25% PEG3350, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
