2OF1
Cryogenic crystal structure of the Staphylococcal Nuclease variant truncated Delta+PHS I92W
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 98 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-12-31 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 60.313, 63.620, 46.727 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.100 - 1.920 |
| R-factor | 0.2181 |
| Rwork | 0.218 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1snc |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.100 | 1.990 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.044 | 0.147 |
| Number of reflections | 14079 | |
| <I/σ(I)> | 11.7 | 4 |
| Completeness [%] | 98.6 | 93.9 |
| Redundancy | 5.15 | 4.68 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 20-30% MPD, 10.5 mM potassium phosphate, 5% glycerol, 5% DMSO, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
