2OEQ
Protein of Unknown Function (DUF964) from Bacillus stearothermophilus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-02 |
Detector | SBC-2 |
Wavelength(s) | 0.97907, 0.97923 |
Spacegroup name | P 1 |
Unit cell lengths | 43.901, 54.353, 59.629 |
Unit cell angles | 69.67, 81.93, 72.57 |
Refinement procedure
Resolution | 34.170 - 2.900 |
R-factor | 0.23164 |
Rwork | 0.222 |
R-free | 0.31437 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.576 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.900 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.106 | 0.513 |
Number of reflections | 10827 | |
<I/σ(I)> | 8.2 | 2.1 |
Completeness [%] | 94.8 | 70.6 |
Redundancy | 4.25 | 2.96 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 295 | 0.2M Potassium dihydrogen phosphate, 15% PEG3350, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |