2OE0
Crystal Structure of Mitochondrial Thioredoxin 3 from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-12-30 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54179 |
Spacegroup name | P 31 |
Unit cell lengths | 49.569, 49.569, 94.423 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.380 - 2.000 |
R-factor | 0.1741 |
Rwork | 0.173 |
R-free | 0.19986 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eru |
RMSD bond length | 0.017 |
RMSD bond angle | 1.453 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.380 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.074 | 0.306 |
Number of reflections | 17467 | |
<I/σ(I)> | 14.8 | 3.6 |
Completeness [%] | 99.7 | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 0.2M ammonium sulfate, 0.1M sodium acetate, 8% PEG 4000, 15% glycerol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |