2ODT
Structure of human Inositol 1,3,4-trisphosphate 5/6-kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-10 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.93 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.330, 39.550, 67.390 |
Unit cell angles | 90.00, 98.40, 90.00 |
Refinement procedure
Resolution | 20.220 - 2.010 |
R-factor | 0.18117 |
Rwork | 0.177 |
R-free | 0.25723 |
Structure solution method | SAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.802 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 20997 | |
<I/σ(I)> | 11 | 3.4 |
Completeness [%] | 95.5 | 77.2 |
Redundancy | 3.4 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 1.6 M Citrate, Tris buffer, 0.1 M, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |