2OD8
Structure of a peptide derived from Cdc9 bound to PCNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 139.280, 139.280, 139.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.700 - 2.800 |
| R-factor | 0.24865 |
| Rwork | 0.247 |
| R-free | 0.28527 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1plq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.081 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.039 | 0.418 |
| Number of reflections | 11287 | |
| <I/σ(I)> | 5 | 2 |
| Completeness [%] | 99.2 | 99.2 |
| Redundancy | 4.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | 1.6 M (NH4)2SO4, Sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
