2OCY
Complex of the guanine exchange factor Sec2p and the Rab GTPase Sec4p
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 160 |
Detector technology | CCD |
Collection date | 2006-02-10 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 92.999, 92.999, 295.909 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 3.300 |
R-factor | 0.25 |
Rwork | 0.250 |
R-free | 0.32500 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.420 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.077 | 0.305 |
Number of reflections | 11817 | |
<I/σ(I)> | 14.5 | 3.3 |
Completeness [%] | 97.7 | 96.7 |
Redundancy | 4.2 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 100 mM sodium acetate (pH 5.0), 200 mM calcium acetate, 20% PEG 400, 10mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 295K |