2OCH
J-domain of dnj-12 from Caenorhabditis elegans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-21 |
Detector | SBC-3 |
Wavelength(s) | 0.979 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 37.664, 51.758, 85.803 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.600 - 1.860 |
R-factor | 0.2204 |
Rwork | 0.219 |
R-free | 0.24720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2O37 PDB ENTRY |
RMSD bond length | 0.017 |
RMSD bond angle | 1.566 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.500 | 1.900 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.052 | 0.601 |
Number of reflections | 7363 | |
<I/σ(I)> | 34.8 | 3.04 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.5 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 2.4 M sodium malonate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |