2OCE
Crystal structure of Tex family protein PA5201 from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-18 |
Detector | MAR CCD 165 mm |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.974, 113.027, 144.186 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.100 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.27900 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.280 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.173 | 0.573 |
Number of reflections | 18579 | |
<I/σ(I)> | 20 | 12.3 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 12.9 | 12.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 277 | 13% PEG 8000, 0.1M Sodium cacodylate pH 6.5, 0.12M Magnesium acetate, 5% Ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 6.50 |