2OAT
ORNITHINE AMINOTRANSFERASE COMPLEXED WITH 5-FLUOROMETHYLORNITHINE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 115.280, 115.280, 186.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.500 * - 1.950 |
| R-factor | 0.206 |
| Rwork | 0.204 |
| R-free | 0.23200 |
| Structure solution method | DIFFERENCE FOURIER TECHNIQUES |
| Starting model (for MR) | 1oat |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.950 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.076 * | 0.297 * |
| Number of reflections | 101836 | |
| <I/σ(I)> | 12.5 | 3.4 |
| Completeness [%] | 97.0 | 94 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.9 | (2S,5S)5FMORN-OAT WAS CO-CRYSTALLIZED FROM 6-10% PEG 6000, 1MM DTT, 120-160 MM NACL, 10-20% GLYCEROL, 50 MM TRICIN, PH 7.9. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 6-10 (%(v/v)) | |
| 2 | 1 | reservoir | 1,4-dithiothreitol | 1 (mM) | |
| 3 | 1 | reservoir | 120-160 (mM) | ||
| 4 | 1 | reservoir | Tricin | 50 (mM) | |
| 5 | 1 | reservoir | glycerol | 15-20 (%(v/v)) |
