Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 |
| Unit cell lengths | 212.308, 212.308, 97.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.500 |
| R-factor | 0.319 |
| Rwork | 0.319 |
| R-free | 0.33800 |
| Structure solution method | MIR |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.910 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.600 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 25597 | |
| <I/σ(I)> | 12.6 | 1 |
| Completeness [%] | 50.4 | |
| Redundancy | 2.7 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.7 | PROTEIN CONCENTRATION 15-20 MG/ML AND 0.05% DODECYLMALTOSIDE.PROTEIN WAS MIXED IN A RATIO OF 4:3 OR 3:2 WITH THE RESERVOIR SOLUTION CONTAINING 100-120 mM AMMONIUM SULFUATE, 23-27% TRIETHYLENE GLYCOL, 100 mM GLYCINE, WITH 1-3 mM GD(CL)3 OR SM(CL)3 AND D2O AS THE SOLVENT. 1 mM (NA3)AU(S2O3)2 WAS SOAKED INTO THE CRYSTAL, pH 3.7, VAPOR DIFFUSION, SITTING DROP |
