2OAL
RebH with bound FAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-08 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97496 |
| Spacegroup name | P 62 |
| Unit cell lengths | 114.725, 114.725, 230.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.900 - 2.100 |
| Rwork | 0.197 |
| R-free | 0.22200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2e4g |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.250 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.900 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 99732 | |
| <I/σ(I)> | 15.8 | 6.6 |
| Completeness [%] | 99.6 | 97.2 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7 | 277 | 1.2 M Na/K phosphate, pH 7.0, hanging drop, temperature 277K |
