2OAJ
Crystal structure of Sro7 from S. cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0781 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 130.520, 130.520, 116.023 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.120 - 2.400 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.26200 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.300 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.108 | 0.430 |
| Number of reflections | 39624 | |
| <I/σ(I)> | 13.7 | 3.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.7 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 40 mM MES pH 6.5, 12% PEG 3350, 200 mM ammonium fluoride, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
