2OA5

Crystal structure of ORF52 from Murid herpesvirus (MUHV-4) (Murine gammaherpesvirus 68) at 2.1 A resolution. Northeast Structural Genomics Consortium target MHR28B.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-09
Detector	ADSC QUANTUM 4
Spacegroup name	C 1 2 1
Unit cell lengths	93.462, 49.393, 56.379
Unit cell angles	90.00, 111.88, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.215
Rwork	0.215
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	COMO (1.2)
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.170
High resolution limit [Å]	2.100	4.510	2.100
Rmerge	0.064	0.051	0.353
Number of reflections	13827
<I/σ(I)>	18.8
Completeness [%]	98.4	99.9	86.7
Redundancy	7.1	6.6	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	80% PEG 400, 100MM MOPS, 100MM NANO3, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K