2O9C
Crystal Structure of Bacteriophytochrome chromophore binding domain at 1.45 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.333, 51.750, 80.437 |
| Unit cell angles | 90.00, 116.29, 90.00 |
Refinement procedure
| Resolution | 19.550 - 1.450 |
| R-factor | 0.1664 |
| Rwork | 0.165 |
| R-free | 0.19204 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ZTU without chromophore or his tag |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.842 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.039 | 0.276 |
| Number of reflections | 56868 | |
| <I/σ(I)> | 12.6 | 3 |
| Completeness [%] | 97.5 | 83.3 |
| Redundancy | 3.7 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.095 M sodium citrate, 19% v/v isopropanol, 19% v/v PEG 4000, 5% V/V glycerol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
