2O99
The crystal structure of E.coli IclR C-terminal fragment in complex with glyoxylate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-02-16 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.538, 81.483, 154.693 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.20661 |
| Rwork | 0.204 |
| R-free | 0.26116 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.861 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.2SSI) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.590 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.060 | 0.352 |
| Number of reflections | 73139 | |
| <I/σ(I)> | 13.7 | 3.9 |
| Completeness [%] | 97.1 | 85.5 |
| Redundancy | 5.93 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 297 | 0.2M Potassium Acetate, pH 7.0, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
