2O8O
Crystal structure of Clostridium histolyticum colg collagenase collagen-binding domain 3B at 1.35 Angstrom resolution in presence of calcium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 93 |
Detector technology | CCD |
Wavelength(s) | 1.28149 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.866, 59.170, 48.801 |
Unit cell angles | 90.00, 100.77, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.350 |
R-factor | 0.1469 |
Rwork | 0.147 |
R-free | 0.20980 |
Structure solution method | AB INITIO |
RMSD bond length | 0.012 |
RMSD bond angle | 0.032 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | |
High resolution limit [Å] | 1.350 | 1.350 |
Number of reflections | 45040 | |
<I/σ(I)> | 1.8 | |
Completeness [%] | 99.8 | 99.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 25-27% PEG 3350, 100mM Sodium acetate, 600mM LiCl, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |