2O7S
Crystal Structure of the A. thaliana DHQ-dehydroshikimate-SDH-shikimate-NADP(H)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 200 |
| Detector technology | AREA DETECTOR |
| Wavelength(s) | 0.9807 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 97.447, 97.447, 116.430 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.780 |
| R-factor | 0.2688 |
| R-free | 0.23340 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.780 |
| Number of reflections | 61503 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | 0.4M K-Na-tartrate, 0.1M sodium citrate (pH 5.6), 2.4M ammonium sulfate, 1 mM shikimate, 33 mM NADP+ soak , VAPOR DIFFUSION, HANGING DROP, temperature 295K |
