2O71
Crystal structure of RAIDD DD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 56.058, 56.058, 64.915 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| Rwork | 0.231 |
| R-free | 0.24100 |
| Structure solution method | SAD |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.052 |
| Number of reflections | 8331 |
| Completeness [%] | 96.9 |
| Redundancy | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 1 ml of protein solution (4-6 mg/ml in 20 mM Tris at pH 8.0, 150 mM NaCl, mixed with 1 ml of a reservoir solution 2M Na/K phosphate at pH 7.0, VAPOR DIFFUSION, HANGING DROP |
