2O6Y
Tyrosine ammonia-lyase from Rhodobacter sphaeroides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.925, 155.626, 164.352 |
| Unit cell angles | 90.00, 94.13, 90.00 |
Refinement procedure
| Resolution | 500.000 - 1.500 |
| Rwork | 0.174 |
| R-free | 0.18900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gkm: Histidine ammonia-lyase from Pseudomonas putida |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.175 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.085 | 0.618 |
| Number of reflections | 652406 | |
| <I/σ(I)> | 12.2 | 2.3 |
| Completeness [%] | 93.3 | 85.9 |
| Redundancy | 5.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.1 M MOPSO (pH 7.0), 7% (w/v) polyethylene glycol 8000, 0.3 M ammonium acetate, 2 mM dithiothreitol, 35 mM cyclohexylbutanoyl-N-hydroxyethylglucamide., VAPOR DIFFUSION, HANGING DROP, temperature 277K |
