2O6W

Crystal Structure of a Pentapeptide Repeat Protein (Rfr23) from the cyanobacterium Cyanothece 51142

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	I 41
Unit cell lengths	106.391, 106.391, 52.419
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.400
Rwork	0.251
R-free	0.26230
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.078
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	16261
<I/σ(I)>	42.7	4.53
Completeness [%]	89.3	61.7
Redundancy	10.8	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	0.192 magnesium acetate, 0.096M sodium cacodylate, 10%(w/v) PEG 8000, 10%(w/v) glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K