2O6S
Structural diversity of the hagfish Variable Lymphocyte Receptors B59
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 295 |
| Detector technology | CCD |
| Collection date | 2006-07-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97175 |
| Spacegroup name | P 1 |
| Unit cell lengths | 30.657, 50.027, 69.642 |
| Unit cell angles | 69.21, 76.99, 86.12 |
Refinement procedure
| Resolution | 19.820 - 1.500 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o6r |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 58682 |
| <I/σ(I)> | 28.7 |
| Completeness [%] | 91.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 33% PEG 1000, 0.1M Bis-Tris, 0.2M Sodium chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
