2O5W
Structure of the E. coli dihydroneopterin triphosphate pyrophosphohydrolase in complex with Sm+3 and pyrophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-01 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.541 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.416, 42.909, 108.328 |
| Unit cell angles | 90.00, 115.19, 90.00 |
Refinement procedure
| Resolution | 19.970 - 2.600 |
| R-factor | 0.2 |
| Rwork | 0.195 |
| R-free | 0.28700 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.152 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.970 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 14304 | |
| Completeness [%] | 99.6 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 298 | 1.3-1.5 AMMONIUM SULFATE, 1% PROPANOL, 3-5 MM DTT, 4MM SODIUM PYROPHOSPHATE, 100MM NA HEPES, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
