2O5N
Crystal structure of a Viral Glycoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.969, 78.178, 62.726 |
Unit cell angles | 90.00, 90.43, 90.00 |
Refinement procedure
Resolution | 62.750 - 2.400 |
R-factor | 0.233 |
Rwork | 0.230 |
R-free | 0.27900 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.318 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.750 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.081 | 0.404 |
Number of reflections | 26191 | |
<I/σ(I)> | 9.7 | 1.54 |
Completeness [%] | 97.5 | 80.6 |
Redundancy | 12.8 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 25 % PEG 2000 MME, 0.1M Tris-HCl, 0.5 % n-dodecyl-b-D-maltoside, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 25 % PEG 2000 MME, 0.1M Tris-HCl, 0.5 % n-dodecyl-b-D-maltoside, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |