2O5C
Structure of E. coli topoisomerase III in complex with an 8-base single stranded oligonucleotide. Frozen in glucose pH 5.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9479 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 102.210, 102.210, 443.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.147 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.050 | 0.215 |
| Total number of observations | 9173 | |
| Number of reflections | 95028 | |
| <I/σ(I)> | 10.4 | 3.6 |
| Completeness [%] | 96.1 | 74.2 |
| Redundancy | 3.6 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 1.5 M (NH4)SO4, 0.1M Sodium citrate, 0.5 M NaCl, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | (NH4)SO4 | ||
| 2 | 1 | 1 | Sodium citrate | ||
| 3 | 1 | 1 | NaCl | ||
| 4 | 1 | 1 | HOH | ||
| 5 | 1 | 2 | (NH4)SO4 | ||
| 6 | 1 | 2 | Sodium citrate | ||
| 7 | 1 | 2 | NaCl | ||
| 8 | 1 | 2 | HOH |
