2O4V

An arginine ladder in OprP mediates phosphate specific transfer across the outer membrane

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	105
Detector technology	CCD
Collection date	2005-12-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97965, 0.97983, 0.96441
Spacegroup name	C 1 2 1
Unit cell lengths	255.130, 142.769, 62.025
Unit cell angles	90.00, 99.49, 90.00

Refinement procedure

Resolution	62.900 - 1.940
R-factor	0.17528
Rwork	0.174
R-free	0.19397
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.154
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHELXS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	62.900
High resolution limit [Å]	1.940
Rmerge	0.067
Number of reflections	147021
<I/σ(I)>	14.8
Completeness [%]	91.1
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Hepes pH 6.5, 50mM K2HPO4, 5% MPD, 7.5-10% PEG8K, VAPOR DIFFUSION, SITTING DROP, temperature 291K
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Hepes pH 6.5, 50mM K2HPO4, 5% MPD, 7.5-10% PEG8K, VAPOR DIFFUSION, SITTING DROP, temperature 291K