2O4A
Crystal Structure of the N-terminal CUT Domain of SATB1 Bound to Matrix Attachment Region DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2006-06-03 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 32.659, 38.318, 40.709 |
Unit cell angles | 71.34, 82.50, 66.63 |
Refinement procedure
Resolution | 22.290 - 1.750 |
R-factor | 0.245 |
Rwork | 0.245 |
R-free | 0.28200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o49 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.040 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.290 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.094 | 0.275 |
Number of reflections | 14958 | |
<I/σ(I)> | 9.2 | 2 |
Completeness [%] | 86.3 | 55.2 |
Redundancy | 2.8 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% PEG 20000, 0.05M TrisHCl, 0.01M magnesium sulfate, 20% ethylene glycol, pH 8.00, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 20000 | ||
2 | 1 | 1 | TrisHCl | ||
3 | 1 | 1 | magnesium sulfate | ||
4 | 1 | 1 | ethylene glycol | ||
5 | 1 | 1 | HOH | ||
6 | 1 | 2 | PEG 20000 | ||
7 | 1 | 2 | TrisHCl | ||
8 | 1 | 2 | magnesium sulfate | ||
9 | 1 | 2 | HOH |