2O42

Crystal Structure of M11L, Bcl-2 homolog from myxoma virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-01-08
Detector	ADSC QUANTUM 210
Spacegroup name	P 31 2 1
Unit cell lengths	73.151, 73.151, 186.875
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.910
R-factor	0.25114
Rwork	0.249
R-free	0.29000
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.966
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.000
High resolution limit [Å]	2.900	2.900
Number of reflections	13415
<I/σ(I)>	22.92	4.596
Completeness [%]	100.0	100
Redundancy	7.6	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	sodium citrate, ammonium sulfate, KCl, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	sodium citrate, ammonium sulfate, KCl, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	sodium citrate, ammonium sulfate, KCl, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K