2O3Y
Crystal Structure of the Homo sapiens Cytoplasmic Ribosomal Decoding Site in Presence of Paromamine Derivative NB30
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-02 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.8730 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 47.940, 49.230, 57.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.700 |
Rwork | 0.245 |
R-free | 0.28200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fqn |
RMSD bond length | 0.005 |
RMSD bond angle | 1.000 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.380 | 37.380 | 2.800 |
High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
Rmerge | 0.086 | 0.037 | 0.387 |
Total number of observations | 1285 | 1336 | |
Number of reflections | 3818 | ||
<I/σ(I)> | 7.8 | 20.6 | 2.7 |
Completeness [%] | 94.7 | 90 | 96.7 |
Redundancy | 3.4 | 3.09 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | Sodium Cacodylate, Potassium chloride, 2-methyl-2,4-pentanediol, hexammine cobalt chloride, spermine tetrahydrochloride , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Sodium Cacodylate | ||
10 | 1 | 2 | spermine tetrahydrochloride | ||
2 | 1 | 1 | Potassium chloride | ||
3 | 1 | 1 | 2-methyl-2,4-pentanediol | ||
4 | 1 | 1 | hexammine cobalt chloride | ||
5 | 1 | 1 | spermine tetrahydrochloride | ||
6 | 1 | 2 | Sodium Cacodylate | ||
7 | 1 | 2 | Potassium chloride | ||
8 | 1 | 2 | 2-methyl-2,4-pentanediol | ||
9 | 1 | 2 | hexammine cobalt chloride |