2O3X
Crystal Structure of the Prokaryotic Ribosomal Decoding Site Complexed with Paromamine Derivative NB30
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9737 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.140, 46.830, 85.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.900 |
Rwork | 0.246 |
R-free | 0.28500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j7t |
RMSD bond length | 0.007 |
RMSD bond angle | 1.234 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.860 | 42.860 | 3.000 |
High resolution limit [Å] | 2.900 | 6.240 | 2.900 |
Rmerge | 0.163 | 0.071 | 0.350 |
Total number of observations | 2105 | 2266 | |
Number of reflections | 3222 | ||
<I/σ(I)> | 8.3 | 32.9 | 3 |
Completeness [%] | 99.4 | 95.3 | 100 |
Redundancy | 6.53 | 5.76 | 6.93 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | Sodium Cacodylate, Potassium chloride, 2-methyl-2,4-pentanediol, spermine tetrahydrochloride, glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Sodium Cacodylate | ||
2 | 1 | 1 | Potassium chloride | ||
3 | 1 | 1 | 2-methyl-2,4-pentanediol | ||
4 | 1 | 1 | spermine tetrahydrochloride | ||
5 | 1 | 1 | glycerol | ||
6 | 1 | 2 | Sodium Cacodylate | ||
7 | 1 | 2 | Potassium chloride | ||
8 | 1 | 2 | 2-methyl-2,4-pentanediol | ||
9 | 1 | 2 | spermine tetrahydrochloride |