2O3W

Crystal Structure of the Homo sapiens Cytoplasmic Ribosomal Decoding Site in presence of paromomycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9000
Spacegroup name	P 21 21 2
Unit cell lengths	47.537, 50.310, 57.397
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.350 - 2.800
Rwork	0.251
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fqn
RMSD bond length	0.006
RMSD bond angle	0.990
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	57.397	57.400	2.950
High resolution limit [Å]	2.800	8.850	2.800
Rmerge	0.085	0.060	0.414
Total number of observations		611	3428
Number of reflections	3672
<I/σ(I)>	5	8.4	1.6
Completeness [%]	99.8	92.1	100
Redundancy	6.2	4.6	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	300	Sodium Cacodylate, Potassium chloride, 2-methyl-2,4-pentanediol, hexammine cobalt chloride, spermine tetrahydrochloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	Sodium Cacodylate
2	1	1	Potassium chloride
3	1	1	2-methyl-2,4-pentanediol
4	1	1	hexammine cobalt chloride
5	1	1	spermine tetrahydrochloride
6	1	2	Potassium chloride
7	1	2	2-methyl-2,4-pentanediol
8	1	2	hexammine cobalt chloride
9	1	2	spermine tetrahydrochloride