2O3J
Structure of Caenorhabditis Elegans UDP-Glucose Dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-15 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.716, 144.092, 92.072 |
| Unit cell angles | 90.00, 123.24, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.880 |
| R-factor | 0.2102 |
| Rwork | 0.209 |
| R-free | 0.26315 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dli |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.114 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.064 | 0.730 |
| Number of reflections | 133254 | |
| <I/σ(I)> | 2.7 | 0.8 |
| Completeness [%] | 98.7 | 90.4 |
| Redundancy | 2.7 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 290 | 200 MM MgCl2, 100 MM BIS-TRIS, PH 5.50, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K |
