2O2T
The crystal structure of the 1st PDZ domain of MPDZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.366, 32.660, 92.803 |
| Unit cell angles | 90.00, 110.68, 90.00 |
Refinement procedure
| Resolution | 29.780 - 2.700 |
| R-factor | 0.22681 |
| Rwork | 0.224 |
| R-free | 0.29131 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.250 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.780 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.078 | 0.513 |
| Number of reflections | 8029 | |
| <I/σ(I)> | 1.93 | |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.5% Jeffamine 2001, 1.1M Sodium Malonate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
