2O2R
Crystal structure of the C-terminal domain of rat 10'formyltetrahydrofolate dehydrogenase in complex with NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-24 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 258.700, 194.300, 97.100 |
| Unit cell angles | 90.00, 108.90, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.20200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2o2q |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.381 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.099 | 0.506 |
| Number of reflections | 226906 | |
| <I/σ(I)> | 12.4 | 3.7 |
| Completeness [%] | 99.2 | 98.4 |
| Redundancy | 5.4 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 288 | 1.6 M AMMONIUM SULPHATE, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
