2O2J
Mycobacterium tuberculosis tryptophan synthase beta chain dimer (apoform)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-26 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.05 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 83.823, 114.966, 159.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.560 |
| R-factor | 0.21441 |
| Rwork | 0.210 |
| R-free | 0.30376 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o2e |
| RMSD bond length | 0.039 |
| RMSD bond angle | 3.112 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.630 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Rmerge | 0.047 | 0.367 |
| Number of reflections | 22608 | |
| <I/σ(I)> | 13.5 | 2 |
| Completeness [%] | 96.9 | 93.4 |
| Redundancy | 2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M Hepes, 2.0 M Ammonium Formate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
