2O1U
Structure of full length GRP94 with AMP-PNP bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 99.330, 109.260, 148.887 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.890 - 2.400 |
R-factor | 0.24537 |
Rwork | 0.243 |
R-free | 0.28888 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | GRP94(73-754d41) |
RMSD bond length | 0.008 |
RMSD bond angle | 1.335 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.890 | 2.350 |
High resolution limit [Å] | 2.230 | 2.230 |
Rmerge | 0.082 | 0.504 |
Number of reflections | 79363 | |
<I/σ(I)> | 22.6 | 3.1 |
Completeness [%] | 99.6 | 98.1 |
Redundancy | 12.4 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 291 | 26-32% v/v PEG400, 150-225mM MgCl2, and 0.1M Bis-tris propane, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |