2O1P
Structure of yeast Poly(A) Polymerase in a somewhat closed state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.217, 108.587, 132.718 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.510 - 2.700 |
| R-factor | 0.19892 |
| Rwork | 0.195 |
| R-free | 0.27508 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fa0 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.318 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | 0.306 |
| Number of reflections | 31665 | |
| <I/σ(I)> | 16.9 | 5.2 |
| Completeness [%] | 99.3 | 97.7 |
| Redundancy | 10.8 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 277 | 20% Peg 8000 100mM magnesium acetate 100mM imidazole pH 6.2 3% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
