2O1B
Structure of aminotransferase from Staphylococcus aureus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9795, 0.9796, 0.9642 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.311, 56.307, 73.401 |
| Unit cell angles | 90.00, 130.31, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.18071 |
| Rwork | 0.178 |
| R-free | 0.22633 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.250 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.079 | 0.197 |
| Number of reflections | 21132 | |
| <I/σ(I)> | 11.6 | 2.8 |
| Completeness [%] | 89.2 | 77.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
