2O18
Crystal structure of a Thiamine biosynthesis lipoprotein apbE, NorthEast Strcutural Genomics target ER559
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9791, 0.9796,0.960 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.320, 70.653, 86.637 |
| Unit cell angles | 75.76, 71.67, 69.46 |
Refinement procedure
| Resolution | 49.740 - 2.200 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.28400 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.060 | 0.253 |
| Number of reflections | 114432 | |
| <I/σ(I)> | 12 | 9 |
| Completeness [%] | 95.0 | 81.4 |
| Redundancy | 1.9 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.15 | 298 | 0.1M PEG 1K, 0.2M LiBr, 0.1M MES, pH 6.15, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
