2O02
Phosphorylation independent interactions between 14-3-3 and Exoenzyme S: from structure to pathogenesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97883 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.330, 72.238, 125.664 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.740 - 1.500 |
| R-factor | 0.151 |
| Rwork | 0.147 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.558 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.600 | |
| High resolution limit [Å] | 1.500 | 10.000 | 1.500 |
| Rmerge | 0.074 | 0.019 | 0.314 |
| Number of reflections | 103943 | ||
| <I/σ(I)> | 16.21 | 66.9 | 3.62 |
| Completeness [%] | 99.5 | 92.5 | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 285 | pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 285K |
