2NZ4
Structural investigation of the GlmS ribozyme bound to its catalytic cofactor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.127, 234.157, 105.003 |
| Unit cell angles | 90.00, 90.65, 90.00 |
Refinement procedure
| Resolution | 34.940 - 2.498 |
| R-factor | 0.22395 |
| Rwork | 0.222 |
| R-free | 0.26897 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.507 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.940 | 2.590 |
| High resolution limit [Å] | 2.498 | 2.498 |
| Rmerge | 0.062 | |
| Number of reflections | 79785 | |
| <I/σ(I)> | 23.2 | 1.1 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.1 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 298 | 11% PEG 8000, 9% DMSO, 0.02M sodium cacodylate pH 6.8, 0.02M magnesium chloride, 0.15M potassium chloride, 0.002M glucosamine 6 phosphate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 10 | 1 | 2 | glucosamine 6 phosphate | ||
| 2 | 1 | 1 | DMSO | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | KCl | ||
| 5 | 1 | 2 | PEG 8000 | ||
| 6 | 1 | 2 | DMSO | ||
| 7 | 1 | 2 | MgCl2 | ||
| 8 | 1 | 2 | KCl | ||
| 9 | 1 | 2 | sodium cacodylate |
